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Product Info Summary
| SKU: | M00449 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PPAR gamma Rabbit Monoclonal Antibody
View all PPAR gamma/NR1C3 Antibodies
SKU/Catalog Number
M00449
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PPAR gamma Rabbit Monoclonal Antibody catalog # M00449. Tested in WB application. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PPAR gamma Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00449)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOFH-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PPAR gamma
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00449 is reactive to PPARG in Human
Applications
M00449 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
73 kDa
Calculated molecular weight
57.62kDa
Background of PPAR gamma/NR1C3
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of PPAR gamma expression in (1) HeLa cell lysate; (2) PC-3 cell lysate; (3) THP-1 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For PPARG (Source: Uniprot.org, NCBI)
Gene Name
PPARG
Full Name
Peroxisome proliferator-activated receptor gamma
Weight
57.62kDa
Superfamily
nuclear hormone receptor family
Alternative Names
CIMT1; NR1C3; NR1C3GLM1; Nuclear receptor subfamily 1 group C member 3; peroxisome proliferator-activated receptor gamma 1; peroxisome proliferator-activated receptor gamma; PPAR gamma; PPARG; PPARG1peroxisome proliferative activated receptor, gamma; PPARG2PPARgamma; PPAR-gamma PPARG CIMT1, GLM1, NR1C31, PPARG2, PPARG5, PPARgamma, PPARG peroxisome proliferator activated receptor gamma peroxisome proliferator-activated receptor gamma|PPAR-gamma|nuclear receptor subfamily 1 group C member 3|peroxisome proliferator-activated nuclear receptor gamma variant 1|peroxisome proliferator-activated receptor-gamma 5|peroxisome proliferator-activated receptor-gamma splicing
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPARG, check out the PPARG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPARG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PPAR gamma Rabbit Monoclonal Antibody (M00449)
Hello CJ!
M00449 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Chen X, Raza SHA, Cheng G, Ma X, Wang J, Zan L. Bta-miR-376a Targeting KLF15 Interferes with Adipogenesis Signaling Pathway to Promote Differentiation of Qinchuan Beef Cattle Preadipocytes. Animals (Basel). 2020 Dec 10;10(12):E2362. doi: 10.3390/ani101223
Species: Qinchuan beef bulls
M00449 usage in article: APP:WB, SAMPLE:BOVINE PREADIPOCYTES, DILUTION:1:1000
Rosiglitazone infusion therapy following minimally invasive surgery for intracerebral hemorrhage evacuation decreases matrix metalloproteinase-9 and blood-brain barrier disruption in rabbits.
Enhanced peroxisomal ?-oxidation metabolism in visceral adipose tissues of high-fat diet-fed obesity-resistant C57BL/6 mice
Scorpion in Combination with Gypsum: Novel Antidiabetic Activities in Streptozotocin-Induced Diabetic Mice by Up-Regulating Pancreatic PPAR
Wang W, Liu L, Dang X, Ma S, Zhang M, Wang K. Bmc Musculoskelet Disord. 2012 Aug 9;13:142. Doi: 10.1186/1471-2474-13-142. The Effect Of Core Decompression On Local Expression Of Bmp-2, Ppar-?? And Bone Regeneration In The Steroid-Induced Femoral H...
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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15 Customer Q&As for Anti-PPAR gamma Rabbit Monoclonal Antibody
Question
We have seen staining in human bone marrow. Do you have any suggestions? Is anti-PPAR gamma Rabbit Monoclonal antibody supposed to stain bone marrow positively?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
From what I have seen in literature bone marrow does express PPARG. From what I have seen in Uniprot.org, PPARG is expressed in subcutaneous adipose tissue, heart, adipose tissue, bone marrow, placenta, cervix carcinoma, macrophage, among other tissues. Regarding which tissues have PPARG expression, here are a few articles citing expression in various tissues:
Adipose tissue, Pubmed ID: 8702406, 9144532
Bone marrow, Pubmed ID: 7787419
Cervix carcinoma, Pubmed ID: 23186163
Heart, Pubmed ID: 9065481
Macrophage, Pubmed ID: 25504154
Placenta, Pubmed ID: 8706692, 9356045, 15489334
Boster Scientific Support
Answered: 2020-04-28
Question
We are currently using anti-PPAR gamma Rabbit Monoclonal antibody M00449 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
The anti-PPAR gamma Rabbit Monoclonal antibody (M00449) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-07
Question
Will M00449 anti-PPAR gamma Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
You can see on the product datasheet, M00449 anti-PPAR gamma Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-06
Question
Will anti-PPAR gamma Rabbit Monoclonal antibody M00449 work for WB with placenta?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
According to the expression profile of placenta, PPARG is highly expressed in placenta. So, it is likely that anti-PPAR gamma Rabbit Monoclonal antibody M00449 will work for WB with placenta.
Boster Scientific Support
Answered: 2019-10-24
Question
Is a blocking peptide available for product anti-PPAR gamma Rabbit Monoclonal antibody (M00449)?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
We do provide the blocking peptide for product anti-PPAR gamma Rabbit Monoclonal antibody (M00449). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-23
Question
I was wanting to use your anti-PPAR gamma Rabbit Monoclonal antibody for WB for human placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human placenta identification?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
It shows on the product datasheet, M00449 anti-PPAR gamma Rabbit Monoclonal antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-11
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-PPAR gamma Rabbit Monoclonal antibody M00449. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-09
Question
Can you help my question with product M00449, anti-PPAR gamma Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00449 anti-PPAR gamma Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-28
Question
Is there a BSA free version of anti-PPAR gamma Rabbit Monoclonal antibody M00449 available?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-PPAR gamma Rabbit Monoclonal antibody M00449 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-16
Question
Is this M00449 anti-PPAR gamma Rabbit Monoclonal antibody reactive to the isotypes of PPARG?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
The immunogen of M00449 anti-PPAR gamma Rabbit Monoclonal antibody is A synthesized peptide derived from human PPAR gamma. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-29
Question
I am interested in using your anti-PPAR gamma Rabbit Monoclonal antibody for cell maturation studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-01-04
Answer
I appreciate your inquiry. This M00449 anti-PPAR gamma Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-01-04
Question
My team were satisfied with the WB result of your anti-PPAR gamma Rabbit Monoclonal antibody. However we have seen positive staining in cervix carcinoma nucleus. cytoplasm. note=redistributed from using this antibody. Is that expected? Could you tell me where is PPARG supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-12-18
Answer
From literature, cervix carcinoma does express PPARG. Generally PPARG expresses in nucleus. cytoplasm. note=redistributed from. Regarding which tissues have PPARG expression, here are a few articles citing expression in various tissues:
Adipose tissue, Pubmed ID: 8702406, 9144532
Bone marrow, Pubmed ID: 7787419
Cervix carcinoma, Pubmed ID: 23186163
Heart, Pubmed ID: 9065481
Macrophage, Pubmed ID: 25504154
Placenta, Pubmed ID: 8706692, 9356045, 15489334
Boster Scientific Support
Answered: 2017-12-18
Question
See attached the WB image, lot number and protocol we used for placenta using anti-PPAR gamma Rabbit Monoclonal antibody M00449. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-11-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-07
Question
I am interested in to test anti-PPAR gamma Rabbit Monoclonal antibody M00449 on human placenta for research purposes, then I may be interested in using anti-PPAR gamma Rabbit Monoclonal antibody M00449 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-07-04
Answer
The products we sell, including anti-PPAR gamma Rabbit Monoclonal antibody M00449, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-07-04
Question
I see that the anti-PPAR gamma Rabbit Monoclonal antibody M00449 works with WB, what is the protocol used to produce the result images on the product page?
E. Kulkarni
Verified customer
Asked: 2013-05-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-05-28
